Remote support from beckman coulter maintaining lab productivity and achieving your goals requires the right hardware and application support when you need it. Upon installation the instrument will have 3 active lasers 405 nm, 488 nm, 638 nm and 10 channels for fluorescence detection in the v2b5r3 configuration. They have been designed and tested to be compliant when used with beckman coulter rotors with the laboratory equipment requirements of applicable regulatory agencies. In this part of our magic inside video series, you will be transported inside of the instrument and follow along the light path, learning how the reduction in the number of light. Christopher a user manual for cytoflex s is at your disposition on the side of the. The cytoflex system can be configured with up to three spatiallyseparated lasers. Cytoflex v2b3r2 flow cytometer has 3 active lasers 405 nm, 488 nm, 638 nm and 7 channels for fluorescence detection. Please explore the resources within the user startup guide. Stanford cytoflex hts user guide 11232015 starting up 1. Its superior sensitivity and resolution throughout all configurations give it the edge over other cytometry systems four times its size.
The laser delays are automatically adjusted by the daily qc system, if required. Log on to the computer and doubleclick to start cytexpert. Sheath fluid filter and sample pump tubing can be replaced by the user no service visit required. Feb 04, 2020 beckman coulter life sciences has introduced the dxflex, europes first ceivd, colour clinical flow cytometry system. The cytoflex s flow cytometer series is an expansion of the. Beckman coulter cytoflex daily qc fluorospheres cytoflex.
Cytoflex hts user guide 11232015 pdf stanford medicine. One of the hallmarks of the cytoflex platform is the ability to upgrade, adding additional functionality to the instrument when experimental needs change. Beckman coulters cytoflex lx flow cytometer brings up to sixlaser instruments to the research community, providing up to 21 fluorescent channels. Cytoflex series instructions for use ifu, document number b49006 5. Cytoflex special procedure, flow cytometer beckman coulter. Cytoflex s series upgrades the cytoflex s is comprised of four different series. Activation key to upgrade the cytoflex v0b5r3 flow cytometer with additional parameters. Nov, 2014 the cytoflex system brings you easily upgradeable detection capabilities for up to 3 lasers and color research flow cytometry right on your bench top.
If login issues occur, then use user beckman coulter with password of password no caps. Beckman coulter cytoflex flow cytometer biocompare product. Standard operating procedure of the cytoflex s flow cytometer. Government users restricted rights as per gsa adp schedule contract with beckman coulter, inc. Questo modello amplia le possibilita di ricerca, con una quarta opzione laser, configurazioni multiple e set di filtri unici che consentono agli investigatori di concentrarsi sulla scienza, non sulla strumentazione. Cytoflex is the new flow cytometer of beckman coulter portfolio.
Is very easy to use it because software is really user friendly. The cytoflex flow cytometer, the first introduction to the cytoflex platform, provides the performance you need in an easy to use system allowing you to focus on the science, not the instrumentation. Cytoflex daily qc fluorospheres may be used for daily verification of the cytoflex. Activation key to upgrade the cytoflex v0b2r2 flow cytometer with additional parameters. Cytoflex sheath fluid beckman coulter life sciences b51503 cytoflex daily ir qc fluorospheres beckman coulter life sciences c06147 versacomp antibody capture beads beckman coulter life sciences b22804 versalyse lysing solution beckman coulter life sciences a09777 dulbeccos phosphate buffered saline corning cellgro 21031cv brilliant stain. We offer specialized instruments for routine or high complexity flow cytometric analysis designed with a deep understanding of the clinical workflow and the needs of the hematopathologist. The cytoflex lx is comprised of two different series. Beckman coulter cytoflex s technical specification.
Cytoflex daily qc fluorospheres consists of a suspension of 3. The cytoflex flow cytometer uses simplified light path, relying on a single bandpass filter versus a combination of dichroic and bandpass filters specifically ordered. Each model is equipped with the lasers and full set of bandpass filters. Cytoflex quick user guide this document gives step by step instructions on how to. Upon installation the instrument will have 3 active lasers 405 nm, 488 nm, 638 nm and 7 channels for fluorescence detection in the v2b3r2 configuration. Beckman coulter cytoflex daily qc fluorospheres cytoflex daily qc. You can add community subscriptions in the search bar that says subscribe to more communities. Certified support specialists are expertly trained to identify, isolate and resolve even the most technical issues over the phone. A series is defined by the lasers that it includes. Follow the instructions below for setting up the pmt voltages.
They are offered in a range of predefined configurations allowing laboratories to purchase only the functionality needed. Having never performed these types of experiments before, i was able to have the instrument set up and detecting 50 nm particles within a very short. Overlay plots contain multiple histograms, dot or density plots in a single plot, allowing the user to compare patterns figure 1. Cytoflex lx flow cytometer the cytoflex lx flow cytometer is an expansion of the cytoflex platform. Beckman coulter life sciences launches europes first ceivd. The manual control options provide the opportunity to interact with the instrument, if needed. That helps us quickly understand the issue and move toward a resolutionavoiding unnecessary wait time for an onsite visit. Beckman coulters cytoflex s benchtop flow cytometer brings up to fourlaser.
For flow cytometry experiments it is often desirable to compare expression patterns between populations or even between samples from different time points or stimulation conditions. Beckman coulter allegra x12 series instructions for use manual. This ensures that you do not run out of sheath fluid during an experiment. For more than 80 years, we have been a trusted partner for laboratory professionals, helping to advance scientific research and patient care. Maintenance perform only the maintenance described in this manual. Our technical call center is your first line of support for resolving technical issues. Introduction certification to ensure full system quality, beckman coulter allegra x12 series centrifuges are manufactured in a registered iso 9001 or 485 facility. Run coulter clenz blue cleaning soluon if cells were. Beckman coulter cytoflex manuals and documents medical. Beckman coulter cytoflex sbenchtop cytometry without compromises for. This model expands research possibilities, with a fourth laser option, multiple configurations and unique filter sets that allows investigators to focus on the science, not the instrumentation.
The cytoflex flow cytometer offers three laser options, 488 nm, 638 nm, and 405 nm. Download any file posted by another user of a community feature that you know, or reasonably should know, cannot be. Cytoflex quick guide beth israel deaconess medical center. The cytoflex model provides the traditional laser palette and a number of channels to accommodate most basic flow cytometry assay needs.
Our team of remote support specialists can help you minimize workflow disruptions and maximize instrument uptime with realtime system insights and deep troubleshooting expertise. Kaluza offline analysis capabilities beckman coulter. Cytoflex startup user guide congratulations on the purchase of your new cytoflex, and welcome to the cytoflex family. Activation key to upgrade the cytoflex v0b3r1 flow cytometer with additional parameters. A new user needs to get an introduction to the flow cytometer by dr. Ensure that the connected icon on the status bar near the bottomleft side of the display is green. Beckman coulter hereby authorizes user to copy those documents published on the world wide web by beckman coulter, for noncommercial use, and only within the users organization. Beckman coulter life sciences is taking actions in the best interests of our associates, customers, and business partners as we navigate the growing threats of the 2019 novel coronavirus disease. Nov 26, 2018 the beckman coulter cytoflex flow cytometer is an excellent instrument that can handle a wide variety of experiments.
Refer to importing and exporting compensation in compensation module of the cytoflex ifu for more information. Dxflex flow cytometer launches in europe beckman coulter. Remote support from beckman coulter beckman coulter. Beckman coulter cytoflex sbenchtop cytometry without compromises for special applications, the cytoflex s flow cytometer series is an expansion of. Beckman coulters vertical angle centrifuge rotors reduce the run time for the application as the particle has only a short distance to travel to pellet. A user manual for cytoflex s is at your disposition on the side of the. What type of issue did you experience with the content on the page. Beckman coulters aquios designer software makes it possible to set up userdefined assays on the aquios cl flow cytometer. Detector configuration of a cytoflex lx equipped with the uv laser. Cytoflex flow cytometer from beckman coulter selectscience.
When a beckman coulter instrument is linked to beckmanconnect, our highly trained service specialists can, at your request, get secure, realtime visibility to your system. Beckman coulters commitment to your research dates back to our origin in 1935. Setup procedure for beckman coulter cytoflex flow cytometer. Cytoflex flow cytometer quick start guide data acquisition important for each new lot number, download the corresponding target value file and refer to step 2. The addition of the 375 nm laser, in a spatially separated discrete beam spot, enables excellent excitation of hoechst, dapi and brilliant uv dyes. The beckman coulter du800 spectrophotometer is a pc controlled system intended for use in quantitative and qualitative analysis of biological and chemical agents using spectrophotometric analysis of the uv and visible light regions of the electromagnetic spectrum. The cytoflex s flow cytometer series is an expansion of the cytoflex platform. The following videos demonstrate the use of kaluza analysis offline software package to read files from a variety of instruments and suppliers. An online resource for manual s and ifus for your beckman coulter products. Six spatially separated lasers allows panels to be spread across the spectrum reducing cross talk and spectral overlap.
Flow cytometers, cell sorters and cell preparation systems. Watch this video on cytoflex flow cytometer special procedure by beckman coulter life sciences. Maintenance other than that specified in this manual should be performed only by beckman coulter service representatives. Technical documents for beckman coulter instrumentation. Beckman coulter life sciences is taking actions in the best interests of our associates, customers, and business partners as we navigate the growing threats of the 2019 novel coronavirus disease covid19. The data was compared to plots and statistics generated in the native package and provided sidebyside comparisons to compare fidelity. Unboxing the beckman coulter cytoflex research flow. Violetbluered vbr series, fully activated with colors or as few as four, activate additional channels in the future. Technical specifications for the cytoflex s near uvvioletbluered. Refer to user manual instructions for data analysis for more detailed.
Guide labware setup gls provides the user specific text and graphical setup instructions such as reagent volume calculations and stepbystep instructions to prepare reagents and place labware for ngs library construction. Measuring samples as small as 65 l and returning results in just 35 seconds, our new vicell metaflex bioanalyte analyzer evaluates ph, po2, pco2, glucose, lactate, electrolytes and more. Cytoflex s 375 nm laser cytoflex s 375 nm laser is a new addition to the cytoflex family of benchtop flow cytometers. Locate the table below for your current series to identify available upgrades. Karen hogg, flow cytometry technical specialist of the university of york shares her experience with beckman coulters service and support. The instrument operates within the wavelength range of 190.
Flow cytometers, reagents, software beckman coulter. The cytoflex s flow cytometer platform expands your research possibilities, with a fourth laser option, multiple configurations and unique filter sets for your science. Changing to plate reader from semiautomac tube loader. No user intervention is required to ensure optimum system performance. Violetbluered vbr series, fully activated with colors or as. A series is defined by the lasers that it contains. We continue to innovate the most sensitive detection technology for researchers, utilizing side scatter technology for nanoparticle detection to. This instrument can be upgraded with a maximum of 5 violet, 5 blue, and 3 red channels.
The ability to perform small particle analysis will be a deal breaker for some. The instrument can be used after 15 minutes of the parcel arriving. It is possible to upgrade within the series but not between different series. B53230, cytoflex daily qc fluorospheres beckman coulter. All computer systems that store data used to make quality decisions or data that will be reported to the fda must be compliant with 21 cfr part 11. Startup kits are available to ensure that when your unit arrives you will be ready to start your experiments.
Beckman coulter life sciences has introduced the dxflex, europes first ceivd, colour clinical flow cytometry system the sensitivity and resolution of the dxflex expands laboratory testing capabilities, especially those focusing on userdefined ass. Cytoflex runs on di water and not shealth fluid or 1x pbs or 10% bleach. The beckman coulter life sciences cytoflex benchtop flow cytometer features up to 3 lasers for color research flow cytometry. Check the sheath fluid level every time you use the cytometer. Beckman coulter hereby authorizes user to copy those documents published on the world wide web by beckman coulter, for noncommercial use, and only within the user s organization. Run the quality control procedure according to the user manual. This model expands research possibilities with up to six lasers and 21 color parameters. Cytoflex flow cytometer quick start guide amrepflow. B53010, cytoflex v2b3r2 flow cytometer 7 detectors, 3.
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